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Exome or Whole Genome Sequencing?

This is one of the most discussed topics especially in Clinical Genomics! Affordability, accuracy, feasibility and of course time consumption - based on these factor mostly, which sequencing technology is more suitable for clinics? Whole Exome Sequencing or Whole Genome Sequencing? (WGS or WES, WGS vs WES) So here's my 2 cents on this discussion! When it comes to DNA sequencing there has always been a raging debate over the choice of Whole Genome Sequencing (WGS) or Whole Exome Sequencing (WXS) for routine use. Whole genome sequencing (WGS), as the name suggests is the process of obtaining the entire genome. In most cases however, this is far from practical and only 95-97% of the genome is covered because it is technically difficult to sequence certain regions of the genome (high GC content, large repeat regions, centromeres, telomeres, etc.) with existing technology. “It’s very fair to say the human genome was never fully sequenced,”  - Craig Venter “The human genome ha...

Phobias: How do I get rid of this irrational fear of dogs?

Dogs are lovely creatures & are adorable. No doubts about that. But if there is one in close radius, I have an anxiety attack. I do everything to avoid getting near them. Also have dreams of dogs chasing me! How do I get rid of this fear? My partner has the same thing for dogs..Her fear is inversely proportional to the size of the dog - the smaller the dog the more petrified she gets. I am a Veterinarian and to me it is irrational but I would not say it is irrational for her or anyone to have such phobias as each of them have a strong underlying reason embedded in our brain's circuitry.  Here are some things you could do. 1. Visit a dog shelter nearby and try to go near the cages and let the dogs sniff your hand, you would be surprised how those poor doggies will be timid and shy and sometimes even afraid of You. Once you have passed level one, u could perhaps try and stroke their neck or shoulder gently. You will automatically see the tail wagging as a sig...

Humble beginnings!

It all starts when you start running towards Biology in your high school, not because you love and live biology but mostly because you loathe mathematics. At least that would be the case with the majority of us. I find no need to confess here as I went on to become a Veterinarian.  I know my blog's name is Ramblings of a Bioinformatician and without the mathematical aptitude, it is futile to wish for specialization in this field (I would equate that to an Aston Martin Vantage with a Citroen C1 engine)! I do not loathe mathematics but somehow fell for this pernicious trend of calling oneself a 'math-person' and 'not-a-math-person'! This psychology and related humbuzz are explained in detail in this article -   "There’s one key difference between kids who excel at math and those who don’t". But then again, there was this unsettled feeling. Having spent those numerous one-hour slots in private internet browsing centres, having dismantled the as...